Itopride belongs to the Benzamide group that inhibits dopamine and has a gastrokinetic effect and used for the treatment of dyspepsia. HPLC and UV method was employed for the estimation of Itopride in three different pharmaceutical formulations. A mixture of Water: Methanol in the ratio of (30:70) (v/v) at a pH of 4.8 in isocratic conditions and separation was achieved on Zodiac C18 column (250 X 4.6 mm, 5μ) at ambient temperature. UV detection was monitored at a wavelength of 258nm. In these conditions, the drug elutes at a retention time of 4.2 min for sample and standard solutions. In UV method, the standard drug was diluted using methanol solvent and maximum absorbance was found to be 258nm and the analysis was carried in this wavelength. Beers law limit was found to be in the range of 50-150µg/ml and 5-30µg/ml for HPLC and UV methods respectively. Retune, Gimate and Itcan (50, 50 and 150mg) brands were used for formulation analysis and the % assay was found to be more than 98% for both the HPLC and UV methods in all the brands in the study.