Development and Validation of HPLC Method for the Estimation of Etoposiide in Pharmaceutical Formulations

Authors

  • Rama Krishna Veni P
  • Rama Krishna V
  • A. Srinivasa Rao
  • K. Vijay

Keywords:

Etoposide, Posid tablets, HPLC, Method development, Validation

Abstract

The development and perhaps validation of a novel, quick, precise, yet efficient Reverse Phase HighPerformance Liquid Chromatographic technique for quantification of etoposide in bulk and pharmaceutical formulations. The mode of this process was isocratic employing analytical Kromasil C18- column(250×4.6mm,5µm), at 25oC with methanol:water: orthophosphoric acid (45:45:10) as mobile phase, at 6.10 pH with 1mL/min rate of flow and UV detection in 282nm. The chromatogram produced as a consequence does have a high resolution and then a lower tailing factor (1.05). For such a wide array of drug doses of 1-5 ppm, the linearity curve exhibited a correlation coefficient -r 2 of 0.999. The precision, accuracy, and specificity of the technique were also assessed. The method's usability was further evaluated with Posid 100mg commercial sample.

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Published

31-12-2017

How to Cite

Veni P, R. K. ., V, R. K., Rao, A. S. ., & Vijay, K. . (2017). Development and Validation of HPLC Method for the Estimation of Etoposiide in Pharmaceutical Formulations. Caribbean Journal of Sciences and Technology, 5(1), 072–079. Retrieved from http://caribjscitech.com/index.php/cjst/article/view/178

Issue

Section

Research Article