Validated RP-HPLCMethod for the Estimation of Amphotericin B in Bulk and Pharmaceutical Dosage Form

Abstract

For fast assessment of Amphotericin B in bulk, pharmaceutical dose format, a basic, choosy, linear, precise, yet accurate RP-HPLC technique was devised, validated. Under ambient temperature, isocratic elution with flow rate of 1 mL/min was performed using Luna C18 column: - 250 x 4.6 mm; 5µ.The mobile phase was composed of 60:30:10 v/v/v of acetonitrile, tetrahydrofuran, and ophosphoric acid - pH 6.0 was amended by Triethylamine. UV detection frequency was 287 nm, and also the sample injection volume was 20µl. Amphotericin B had a retention duration of 7.722 minutes.The percent recovery ranged from 99.60 to 100.42 percent. The percent RSD for the method's accuracy and precision was determined to be lower than 2%. The technique was verified in accordance with the International Conference on Harmonization (ICH) criteria. The approach was fruitfully then used analyze Amphotericin B in bulk samples and formulations on a regular basis