SELECTIVITY OF COLUMN PACKING TO PORPHYRIN ELUTION

Abstract

The selectivity of column packings to porphyrin elution was investigated. A total of eighteen different column packings by column length and column weight ratios (comprised of three existent and fifteen novel column packings) were investigated for their relative elution of nickel and vanadium porphyrins from ten outcrop sediment samples by column chromatography using silica gel and alumina as the stationary phase. Column packings by column length ratio, AS (1:1) and SA (1:1) eluted the maximum concentration of nickel porphyrin, 790mmole with a mean of 521±55.42 and vanadium porphyrin, 152mmole with a mean concentration of 92.56 ±12.56 while the column packings by weight ratio, SA (1:2) and AS (1:2) eluted the minimum concentration of nickel porphyrin, 9mmole with a mean concentration of 27±6.02 and vanadium porphyrin, 10mmole with a mean concentration of 25.78± 5.16. Some of the novel column ratios investigated eluted significantly higher concentrations of the porphyrins than the three existing column ratios used by different workers at 95% confidence limit.