High Resolution Liquid Chromatography Mass Spectrometry - A Tool for Structural Elucidation of Seed Metabolites of Abrus Precatorius (L.)

DOI: https://doi.org/10.55434/CBI.2019.7106

Abstract

Plant metabolites with therapeutic values provide enormous scope for novel drug leads. In this context several traditional medicinal plants are targeted for phytochemicals identification and characterization worldwide. The current investigation was undertaken to identify metabolites present in seeds of Abrus precatorius (L.), a medicinal plant with potent anti-inflammatory, antimicrobial, antioxidant, antifertility, and antispermatogenic activity. Ethanol extracted seed metabolites were identified by LC-ESI-Q-TOF-MS system equipped with a G4220B pump, G4226A auto sampler and G1316C, and a diode array detector. Metabolites profiling yielded large sets of data were analyzed by METLIN Mass Spectral Database. Metabolomics analysis revealed presence of Desethyletomidate, Butabarbital, 4-aminohippurate, 20α-Dihydroprogesterone glucuronide, Phenethylamine, Etomidate, Oxyquinoline, Norfloxacin, Phenylethylmalonamide (PEMA), and Dihydro streptomycin with typical plants metabolites. The masses of these uncommon metabolites were 216.08, 212.11, 222.09, 492.27, 121.09, 244.12, 145.05, 319.12, 206.10 and 567.28 respectively. These metabolites contribute to various drug preparations used to treat human ailments.